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Novoprotein t7 high yield rna transcription kit
Production and characteristics of PEDV‐S mRNA and saRNA vaccines. (a) PEDV‐S saRNA vaccine design schematic. (b) Average particle size and PDI of <t>RNA‐LNPs</t> by dynamic light scattering. (c) qRT‐PCR quantification of the <t>transcription</t> efficiency in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (d) Western blotting analysis PEDV‐S protein expression in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (e) Densitometric quantification of PEDV‐S expression relative to β‐tubulin based on the blots of (d). Results in panels (c) and (e) were shown as mean ± SD of three independent experiments.
T7 High Yield Rna Transcription Kit, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t7+high+yield+rna+transcription+kit/kit+reverse+transcription/pmc13147209-39-26-31
Average 86 stars, based on 1 article reviews
t7 high yield rna transcription kit - by Bioz Stars, 2026-09
86/100 stars

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1) Product Images from "A Self‐Amplifying RNA Lipid Nanoparticle (saRNA‐LNP) Vaccine Provides Effective Protection Against Porcine Epidemic Diarrhea"

Article Title: A Self‐Amplifying RNA Lipid Nanoparticle (saRNA‐LNP) Vaccine Provides Effective Protection Against Porcine Epidemic Diarrhea

Journal: Transboundary and Emerging Diseases

doi: 10.1155/tbed/3115893

Production and characteristics of PEDV‐S mRNA and saRNA vaccines. (a) PEDV‐S saRNA vaccine design schematic. (b) Average particle size and PDI of RNA‐LNPs by dynamic light scattering. (c) qRT‐PCR quantification of the transcription efficiency in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (d) Western blotting analysis PEDV‐S protein expression in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (e) Densitometric quantification of PEDV‐S expression relative to β‐tubulin based on the blots of (d). Results in panels (c) and (e) were shown as mean ± SD of three independent experiments.
Figure Legend Snippet: Production and characteristics of PEDV‐S mRNA and saRNA vaccines. (a) PEDV‐S saRNA vaccine design schematic. (b) Average particle size and PDI of RNA‐LNPs by dynamic light scattering. (c) qRT‐PCR quantification of the transcription efficiency in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (d) Western blotting analysis PEDV‐S protein expression in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (e) Densitometric quantification of PEDV‐S expression relative to β‐tubulin based on the blots of (d). Results in panels (c) and (e) were shown as mean ± SD of three independent experiments.

Techniques Used: Vaccines, Quantitative RT-PCR, Transfection, Western Blot, Expressing

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Polymerase Chain Reaction:

Article Title: Anti-CLEC7A nanobody in situ engineering promotes amyloid-β oligomers clearance by CAR-microglia to alleviate Alzheimer's disease pathology in mice.
Article Snippet: Chimeric antigen receptor microglia (CAR-M)-mediated amyloid-β oligomers (AβO) phagocytosis shows great promise in Alzheimer's disease (AD) treatment, however, the limited AβO degradation of CAR-M compromises their anti-AβO potency.. This work here reports an in situ engineered agonistic anti-C-type lectin domain containing 7 A (CLEC7A) nanobody to accelerate AβO degradation of CAR-M, augmenting their anti-AβO efficacy.. Specifically, with the intranasal-delivered microglia-targeting lipid nanoparticles (LNP), this work generates an AβO-specific degradation-potentiated CAR-M by introducing dual mRNAs encoding AβO-specific CAR and antiCLEC7A nanobody into the cerebral microglia.

Purification:

Article Title: Anti-CLEC7A nanobody in situ engineering promotes amyloid-β oligomers clearance by CAR-microglia to alleviate Alzheimer's disease pathology in mice.
Article Snippet: Chimeric antigen receptor microglia (CAR-M)-mediated amyloid-β oligomers (AβO) phagocytosis shows great promise in Alzheimer's disease (AD) treatment, however, the limited AβO degradation of CAR-M compromises their anti-AβO potency.. This work here reports an in situ engineered agonistic anti-C-type lectin domain containing 7 A (CLEC7A) nanobody to accelerate AβO degradation of CAR-M, augmenting their anti-AβO efficacy.. Specifically, with the intranasal-delivered microglia-targeting lipid nanoparticles (LNP), this work generates an AβO-specific degradation-potentiated CAR-M by introducing dual mRNAs encoding AβO-specific CAR and antiCLEC7A nanobody into the cerebral microglia.

In Vitro:

Article Title: Anti-CLEC7A nanobody in situ engineering promotes amyloid-β oligomers clearance by CAR-microglia to alleviate Alzheimer's disease pathology in mice.
Article Snippet: Chimeric antigen receptor microglia (CAR-M)-mediated amyloid-β oligomers (AβO) phagocytosis shows great promise in Alzheimer's disease (AD) treatment, however, the limited AβO degradation of CAR-M compromises their anti-AβO potency.. This work here reports an in situ engineered agonistic anti-C-type lectin domain containing 7 A (CLEC7A) nanobody to accelerate AβO degradation of CAR-M, augmenting their anti-AβO efficacy.. Specifically, with the intranasal-delivered microglia-targeting lipid nanoparticles (LNP), this work generates an AβO-specific degradation-potentiated CAR-M by introducing dual mRNAs encoding AβO-specific CAR and antiCLEC7A nanobody into the cerebral microglia.

Article Title: Loss of Function of the Zxdb Gene Leads to a Decrease in the Decidualization Rate and Number of Pups Born in Mice by Affecting the Expression of the Cell Adhesion Molecules.
Article Snippet: .. Guide RNA sequence1: 5′-ACGCCGAGAUGGAAAUCCCG-3′; Guide RNA sequence2: 5′-GGGACCGCAGACAGACAGCG-3′; sgRNAs were prepared by in vitro transcription using the T7 High Yield RNA Transcription kit (Novoprotein, E131, Suzhou, China). ..

Article Title: TRIM13 in situ engineering boosts anti-inflammatory capacity of CAR-Ms for liver fibrosis therapy
Article Snippet: .. In vitro transcription was performed with a T7 High Yield RNA Transcription Kit, and the resulting transcripts were capped using a Cap 1 Capping System (both from Novoprotein). .. The ELISA kits were purchased from Wuhan Servicebio Technology Co., Ltd.

Article Title: Loss of Function of the Zxdb Gene Leads to a Decrease in the Decidualization Rate and Number of Pups Born in Mice by Affecting the Expression of the Cell Adhesion Molecules
Article Snippet: .. Guide RNA sequence1: 5′-ACGCCGAGAUGGAAAUCCCG-3′; Guide RNA sequence2: 5′-GGGACCGCAGACAGACAGCG-3′; sgRNAs were prepared by in vitro transcription using the T7 High Yield RNA Transcription kit (Novoprotein, E131, Suzhou, China). ..

Article Title: TRIM13 in situ engineering boosts anti-inflammatory capacity of CAR-Ms for liver fibrosis therapy.
Article Snippet: .. In vitro transcription was performed with a T7 High Yield RNA Transcription Kit, and the resulting transcripts were capped using a Cap 1 Capping System (both from Novoprotein). .. The ELISA kits were purchased from Wuhan Servicebio Technology Co., Ltd.

Article Title: Influenza mRNA vaccine with engineered panhandle-forming UTRs provides potent, dose-sparing protection against seasonal influenza viruses.
Article Snippet: .. T7 High Yield RNA Transcription Kit (Novoprotein, China) were used to perform the in vitro transcription according to the manufacturer’s instructions by using linearized DNA as templates. .. In this research, 1-methylpseudouridine-5’-triphosphate (Novoprotein, China) was used instead of uridine triphosphate for mRNA synthesis.

Plasmid Preparation:

Article Title: Synthetic cleavage-resistant TREM2 boosts macrophage efferocytosis to treat inflammatory diseases.
Article Snippet: To detect TREM2 expression, BMDMs were stained with a TREM2 antibody (Thermo Fisher Scientific, MA5-28223) for flow cytometric analysis. .. The plasmid encoding CRT was linearized by restriction enzyme digestion and transcribed into mRNA by the T7 High Yield RNA Transcription Kit (Novoprotein, E131-01A). .. A 5 ′ cap was added by the Cap 1 Capping System (Novoprotein, M082-01A).



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Production and characteristics of PEDV‐S mRNA and saRNA vaccines. (a) PEDV‐S saRNA vaccine design schematic. (b) Average particle size and PDI of <t>RNA‐LNPs</t> by dynamic light scattering. (c) qRT‐PCR quantification of the <t>transcription</t> efficiency in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (d) Western blotting analysis PEDV‐S protein expression in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (e) Densitometric quantification of PEDV‐S expression relative to β‐tubulin based on the blots of (d). Results in panels (c) and (e) were shown as mean ± SD of three independent experiments.
T7 High Yield Rna Transcription Kit, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t7+high+yield+rna+transcription+kit/kit+reverse+transcription/pmc13147209-39-26-31
Average 86 stars, based on 1 article reviews
t7 high yield rna transcription kit - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

Image Search Results


Production and characteristics of PEDV‐S mRNA and saRNA vaccines. (a) PEDV‐S saRNA vaccine design schematic. (b) Average particle size and PDI of RNA‐LNPs by dynamic light scattering. (c) qRT‐PCR quantification of the transcription efficiency in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (d) Western blotting analysis PEDV‐S protein expression in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (e) Densitometric quantification of PEDV‐S expression relative to β‐tubulin based on the blots of (d). Results in panels (c) and (e) were shown as mean ± SD of three independent experiments.

Journal: Transboundary and Emerging Diseases

Article Title: A Self‐Amplifying RNA Lipid Nanoparticle (saRNA‐LNP) Vaccine Provides Effective Protection Against Porcine Epidemic Diarrhea

doi: 10.1155/tbed/3115893

Figure Lengend Snippet: Production and characteristics of PEDV‐S mRNA and saRNA vaccines. (a) PEDV‐S saRNA vaccine design schematic. (b) Average particle size and PDI of RNA‐LNPs by dynamic light scattering. (c) qRT‐PCR quantification of the transcription efficiency in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (d) Western blotting analysis PEDV‐S protein expression in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (e) Densitometric quantification of PEDV‐S expression relative to β‐tubulin based on the blots of (d). Results in panels (c) and (e) were shown as mean ± SD of three independent experiments.

Article Snippet: The transcription reaction consisted of linearized template DNA, N1‐methylpseudouridine‐5 ′ ‐triphosphate (Novoprotein, China), CAP GAU m7G(5 ′ )ppp(5")(2 ′ 0MeA)pU (SYNTHGENE, China), and components from the T7 High‐Yield RNA Transcription kit (Novoprotein, China).

Techniques: Vaccines, Quantitative RT-PCR, Transfection, Western Blot, Expressing