t7 high yield rna transcription kit (Novoprotein)
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T7 High Yield Rna Transcription Kit, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t7+high+yield+rna+transcription+kit/kit+reverse+transcription/pmc13147209-39-26-31
Average 86 stars, based on 1 article reviews
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1) Product Images from "A Self‐Amplifying RNA Lipid Nanoparticle (saRNA‐LNP) Vaccine Provides Effective Protection Against Porcine Epidemic Diarrhea"
Article Title: A Self‐Amplifying RNA Lipid Nanoparticle (saRNA‐LNP) Vaccine Provides Effective Protection Against Porcine Epidemic Diarrhea
Journal: Transboundary and Emerging Diseases
doi: 10.1155/tbed/3115893
Figure Legend Snippet: Production and characteristics of PEDV‐S mRNA and saRNA vaccines. (a) PEDV‐S saRNA vaccine design schematic. (b) Average particle size and PDI of RNA‐LNPs by dynamic light scattering. (c) qRT‐PCR quantification of the transcription efficiency in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (d) Western blotting analysis PEDV‐S protein expression in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (e) Densitometric quantification of PEDV‐S expression relative to β‐tubulin based on the blots of (d). Results in panels (c) and (e) were shown as mean ± SD of three independent experiments.
Techniques Used: Vaccines, Quantitative RT-PCR, Transfection, Western Blot, Expressing
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